The maximum available sampling rate of 5000 Hz should be used. A high sampling rate is required to make sure that the gradient artifact is captured with sufficient temporal resolution to perform a good gradient artifact correction.
The amplitude resolution determines the operating range for the analogue to digital converter (ADC). A large operating range is needed to record the full amplitude of the gradient artifact; an insufficient dynamic range will result in ADC saturation. For the BrainAmp MR plus and BrainAmp ExG MR you have several options available, we recommend using a resolution of 0.5 µV/bit (operating range ± 16 mV) for EEG recordings in the scanner. The BrainAmp MR has a non-changeable resolution of 0.5 µV/bit.
The other resolution options available for the BrainAmp MR plus and BrainAmp ExG MR can be used under exceptional circumstances. A resolution 0.1 µV/bit can be used for your EEG channels if you are recording in a scanner that has small gradient amplitudes that fit within the reduced amplitude range achieved with a resolution of 0.1 µV (±3.2 mV). A resolution of 10 µV/bit (operating range ± 160 mV) can be considered for the ECG channel if you are experiencing problems with saturation in this channel. The ECG channel is more prone to saturation because the lead wire is longer, thus creating a larger loop between the recording electrode and the reference electrode.
We recommend that you always do the first pilot recording with the standard 0.5 µV/bit resolution for all channels. Then check all channels for saturation and decide if any changes should be made. Further information on how to check the data for saturation can be found in our EEG-fMRI webinar.
We recommend a low cutoff filter with a time constant of 10 s. In the BrainAmp MR plus and BrainAmp ExG MR you also have the option to record in DC (direct current) mode, this means that no low cutoff filter is applied. DC mode should only be used if you are interested in the very slow components of the EEG signal.
In case you do want to do DC recordings, there are a few things you should consider. The DC value of the EEG is the baseline around which the oscillatory signal fluctuates, this can change over time resulting in what is known as DC drift. This baseline can drift beyond the range of the amplifier and this results in DC saturation. This can be handled by performing a DC correction, however, there are two problems associated with this. The first is that DC correction might be forgotten, resulting in the loss of data from the saturated channel (the same as in a lab environment). Second, a DC correction results in an offset in the data which will influence the gradient artifact template used during the artifact handling process.
We recommend a high cutoff of 250 Hz. This is an amplifier hardware low pass and the purpose is to limit the gradient artifact before entering the ADC. While this is beneficial for EEG recording, keep in mind that this could be a limiting factor for signals such as EMG, where a broader signal bandwidth might be necessary. With a high cutoff filter of 250 Hz the available bandwidth is approximately 125 Hz. If you would like to do EMG measurements, you should consider whether your EMG response of interest can be seen within this range. If the EMG frequency that you are interested in is higher than 125 Hz you could try using a higher cutoff filter, however, note that this will result in more high frequency noise in the data which will influence how useful the recorded signal will be. Pilot testing your study is the best way to investigate the effects of the amplifier settings on your EMG data.
Correct series resistor values should be entered in the Recorder workspace to prevent false impedance readings. These values can be found on the labels on the connector box of the BrainCap MR, or on the data sheet that is delivered with the cap. A standard BrainCap MR has the following resistor values:
- EEG channels have resistors of 10 kOhm (5 kOhm at the electrode tip and 5 kOhm at the connector box)
- ECG channels have resistors of 20 kOhm (15 kOhm at the electrode tip and 5 kOhm at the connector box)
However, you should check this information before entering it in the Recorder workspace in case you have an older, or customised, BrainCap MR.
Raw Data Saving filters should be disabled. Low pass filters might obscure ADC saturation, and the induced phase shift, especially for high pass filter, may hinder gradient artifact correction.